2012年12月1日 星期六

Elimination of Mycoplasma Contamination in Cell Cultures【萊恩生物科技有限公司04-25239639】ABMgood台灣總代理-黴漿菌偵測套組




黴漿菌偵測套組【萊恩生物科技有限公司04-25239639】貨號G238
產品特點:
1. 100ul細胞培養液就可以測量是否含有黴漿菌污染。不須萃取細胞DNA
2. ready-to-use primer混合液
3. 測量70種以上mycoplasma，靈敏度超高。
4. 包括positive control及internal control。
5. 快速反應，2個小時得知黴漿菌污染。
Features
-direct addition of cell culture supernatant to PCR reaction — no DNA isolation/purification steps required.
-ready-to-use primer mix — reduces variability.
-able to detect over 70 types of mycoplasma species – high sensitivity.
-included positive control to verify negative results.
-rapid results in 2 hours.
黴漿菌”(Mycoplasma) 偵測套組,應用PCR原理, 提供快速及高靈敏度2~ 5 f g/DNA的方法,只要100ul的細胞培養液,可偵測潛在性的mycoplasma (Mycoplasma, Acholeplasma及Ureaplasma)污染。每組kit包括100個反應 (1次PCR反應體積以50ul為例)
引用文獻References
Daniella C. Bartholomeu et al., J. Immunol., Jul 2008; 181: 1333 - 1344.
Mycoplasma detection is often difficult due to its lack of visable appearance, therefore it can be afflicting your valuable cell and affecting your results without your knowledge. Cells contaminated by mycoplasma species can have changes in proliferation, metabolism, gene synthesis and processing, and even adhersion properties. The solution for quick and easy mycoplasma detection is ABM's PCR Mycoplasma Detection Kit.
The PCR Mycoplasma Detection Kit allows for fast and reliable identification of mycoplasma contamination in cell cultures. Mycoplasma DNA in the cell culture supernatant is amplified via PCR and visualized using gel electrophoresis. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this PCR Mycoplasma Detection Kit a convenient tool for routine examination of cell cultures and media.
詳細產品資訊，請登錄www.abmgood.com 查詢  



【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com


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Mycoplasma 黴漿菌快速PCR鑑定。應用PCR原理, 快速及高靈敏度2~5 fg/DNA的方法,偵測潛在性的mycoplasma污染。準確度高達95%以上。SBA代理商萊恩生物科技有限公司。




注意!! 注意!!

您細胞長不好? 實驗做不出來? 因為細胞有Mycoplasma污染了!!
黴漿菌偵測套組, 應用PCR原理, 提供快速及高靈敏度2~ 5 f g/DNA的方法,偵測潛在性的mycoplasma污染。 涵蓋三種mycoplasmatales (mycoplasma, Acholeplasma, 及Ureaplasma)皆可測量到, 準確度高達95%以上。
來自真核及細菌DNA不會由此被測量到,有效監測潛在性的黴漿菌汙染。
產品特點
○可測量2~5pg mycoplasma DNA, 準確度高達95%以上。
○涵蓋三種mycoplasmatales (mycoplasma, Acholeplasma, 及
Ureaplasma)皆可測量到。
○內附有positive control DNA, internal control DNA,
Primer/dNTP mix,及buffer。再贈送您500支的PCR Tube。
○不會測量到真核及細菌DNA。
原廠連結: http://www.Southernbiotech.com

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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【萊恩生物科技有限公司04-25239639】CO2 細胞培養箱或水浴槽T-Pro Aqua EZ Clean,去黴防菌抑菌劑。 可有效預防污染。台灣代理經銷商。


CO2 細胞培養箱或水浴槽去黴抑菌劑。T-Pro Aqua EZ Clean,
可有效預防細胞污染。
台灣代理經銷商。【萊恩生物科技有限公司04-25239639】





上圖為水中雜菌濃縮後塗盤，再置入disc 37℃培養72小時。

上為control (disc僅含有ddH2O)
左下為500X濃度
右下為200X濃度
CO2 細胞培養箱或水浴槽是培養細胞實驗室必須的設備，這些設備常常也是細菌或黴菌等汙染物適合生長的環境，所以我們會建議CO2 細胞培養箱或水浴槽等這些設備，每14天或每月最少清洗消毒一次。

特色：
l 非揮發性。
l 對於細菌或黴菌具抑菌效果。
l 操作簡單可用於CO2 細胞培養箱水盤或水浴槽。
l 抑菌時間可維持2~4週。
操作指南：
用於CO2 細胞培養箱水盤：(200X)
每1公升 無菌水添加5ml T-Pro Aqua EZ Clean，請每2~4週更換水盤中之無菌水並添加T-Pro Aqua EZ Clean，可有效預防污染。
用於水浴槽：(500X)
每次注入水浴槽每1公升 水添加2ml T-Pro Aqua EZ Clean，可有效預防污染抑菌時間可維持2~4週，請每2~4週清洗水浴槽並重新加入水至水浴槽且含有T-Pro Aqua EZ Clean，可有效預防污
上圖為水中雜菌濃縮後塗盤，再置入disc 37℃培養72小時。
上為control (disc僅含有ddH2O)
左下為500X濃度
右下為200X濃度




【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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Biocidal ZFTM WAK-ZF-1, 1 liter spray bottle噴霧式去黴/ 抗微生物劑 細胞培養設備/ 無菌操作台去黴抗污劑

Biocidal ZFTM
Biocidal ZFTM

噴霧式去黴/ 抗微生物劑
WAK-ZF-1 1 liter spray bottle
1. 無臭無味專為細胞培養實驗室設計, 可去除微生物 / 黴菌 / 孢子(also spore forming units)/ 病毒
2. 提供雙重保護, 活性化微生物成份無揮發性, 可保護 細胞培養遭微生物污染也不會借由空氣侵入細胞, 因此抗污劑可保護細胞培養過程
　
使用方法 : 可處理滅菌/ 培養設備, 每14天處理一次
( spray 於設備表面後自然風乾勿擦拭 ), 亦可處理細胞培養 Flask 表面, 但不可接觸 Flasks 內部
每瓶 NT$ 4,500 一次購2 瓶 NT$ 8,500
 
【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com


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黴漿菌除去試劑Mycoaway，G398，其在市場上具有競爭力，其可移除超過70種以上Mycoplasma species。僅需要4次處理。1:1,000倍稀釋為治療。10,000倍稀釋為預防。

Mycoaway G398 Mycoplasma Elimination Cocktail
Cat.No.:
G398
Quantity:
2 x 1.0ml (濃縮)
1:1,000倍稀釋為治療。10,000倍稀釋做為預防。
Mycoaway 黴漿菌除去試劑產品介紹:
黴漿菌污染細胞，會誘導細胞內之蛋白質表現；例如染色體畸變(chromosome aberrations)，造成細胞型態改變及影響細胞生長。因此Abmgood發展一系列黴漿菌除去(Mycoplasma Elimination Cocktail)試劑，Mycoaway。Abmgood提供之黴漿菌除去試劑Mycoaway，其在市場上具有競爭力，其可移除超過70種以上Mycoplasma species。僅需要4次處理 (2週時程)。1:1,000倍稀釋為治療。10,000倍稀釋做為預防。Mycoaway 在經由基因點陣分析中，不會造成細胞毒理反應。是黴漿菌除去的最佳選擇。
Mycoplasma contamination is one of the most challenging problem of daily cell culture. Commonly used antibiotics in cell culture are not effective against mycoplasma contamination. Mycoplasma can damage precious cell lines by inducing cellular changes, such as chromosome aberrations, changes in cell morphology and cell growth. For this reason, scientists at abm have developed qPCR-based assay for mycoplasma contamination (PCR Mycoplasma Detection Kit (G238)) and Mycoplasma Elimination Cocktail (Mycoaway). Compared to similar products on the market, our Mycoaway is much more potent that can eliminate over 50 different species of mycoplama contamination with only 4 treatment. In addition, the drug showed no toxicity to any cell under treatment as determent by gene array assay.
產品特點:
1. 1:10,000倍稀釋預防。直接添加於細胞培養液中。
2. 可移除超過70種以上Mycoplasma species。
3. 僅需要4次處理 (2週時程)。
4. 經由基因點陣分析中，不會造成細胞毒理反應。是黴漿菌除去的最佳選擇。
-direct addition of cocktail to cell culture medium – no change to routine cell culture methods needed.
-effectively eliminates over 70 types of mycoplasma species – broad range.
-not cytotoxic to most mammalian cell lines.
-eliminates mycoplasma in less than 2 weeks (4 cell passages).
Storage
Store at -20°C
Data Sheet
Notes
Mycoplasma contamination detection and mycoplasma elimination customer service are also provided, please refer to the Mycoplasma Detection and Elimination Service page for details.
Mycoplasma操作手冊:
Mycoplasma Elimination Protocol
1. a) For adherent cells:
i. Trypsinize cells of 80- 90% confluency and check for complete detachment.
ii. Centrifuge cells at 1500rpm for 10mins. Discard the supernatant, then resuspend cells using appropriate amount of complete culture medium. Make sure to break all visible cell clumps.
b) For suspension cells:
i. Prepare cell suspension using usual method to a cell density of 1X 106 cells/ml in complete culture medium.
2. Add Mycoplasma Elimination Cocktail at a 1:1000 dilution (1μl of cocktail per 1ml of medium) to cell suspension from step 1.a.ii. or 1.b.i. and mix carefully.
3. Transfer the mixture from step 2 to a sterile culture dish and incubate at usual conditions for 3 days.
4. Repeat steps 1-3 for 3 more times or 4 cell passages. The culture should be mycoplasma free after the final treatment.
5. Confirm the success of mycoplasma elimination using the Mycoplasma PCR Detection Kit (Cat No. G238).
Recommendations for Optimal Results
• Two weeks after treatment, test the cell culture for mycoplasma using the Mycoplasma PCR Detection Kit (Cat No. G238) to ensure that fresh infections do not arise.
• Discard all media used for contaminated cell cultures. Use fresh media in order to prevent future mycoplasma infections due to contaminated media.


【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com
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Abmgood 廣泛型抗生素添加劑，BacAway，貨號G285。有效預防革蘭氏陰性菌及革蘭氏陽性菌之污染。

BacAway, G285
廣泛型抗生素添加劑，有效預防革蘭氏陰性菌及革蘭氏陰
性菌之污染。
Cat.No.:
G285
Quantity:
2 x 1.25ml
Description
Bacterial contamination is often associated with long-term culture of primary cells, stable cell lines, and stem cells. It is often frustrating that cells got contaminated after weeks or months of clone selection or precious establishment of primary cells. Most antibiotics like Ampicillin and others found in most labs can not solve the problems, leading to loss of precious cells. To this end, scientists at ABM have successfully developed a new formulation of antibiotics that can kill any bacteria associated with cell culture. The reagent is so effective that you will once-for-all worry-free when culturing precious cells.
Storage
This product is stored at -20°C , and is stable for 60 months if properly stored. Avoid freeze-thaw cycles by making aliquots upon first thawing this product.
Data Sheet

Protocol
Prepare 1. treatment medium by adding BacAway to sterile cell culture me­dium in a 1:250 dilution.
Wash contaminated cells three times using sterile medium.2.
Add3. treatment medium to the cell culture. Replace with new treament me­dium after 3 hours and 7 hours.
After incubating the cell culture overnight, visually examine the medium. If 4. bacteria are still visible under the microscope, continue to 5. If not, continue to 7.
Trypsinize and centrifuge the cells. Carefully discard the supernatant, then 5. re-seed the cells in a new culture vessel containing treatment medium.
Incubate the cells for 6-7 hours, then replace the medium with new 6. treatment medium.
After incubating overnight, replace the medium with new 7. treatment medium once per day for one week. This will completely eliminate any remaining bacteria.

【供應廠商】萊恩生物科技有限公司
【公司電話】04-2523-9639
【業務專員】陳俊豪 0937-229803
【電子信箱】biolion0819@gmail.com

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#JT90-002黴漿菌噴霧劑。T-Pro MycoClean spray 是可以有效去除黴漿菌、細菌或病毒之污染。抑菌、清潔、去污噴劑一瓶搞定。

(NEW) MycoClean spray

T-Pro MycoClean spray


簡介：

全世界約60~70%的細胞培養被黴漿菌污染，黴漿菌是最小的且可自行複製的生物，大小約0.1~0.8um，它無細胞壁，故部份抗生素如盤尼西林對它無效，在酒精、乙醛及加熱時，可抑制其繁殖，自然界中黴漿菌各處均有，實驗室培養箱、無菌操作台等也不例外。

細胞培養箱的污染源經常是外溢的污染培養液，這些培養液即使乾了，黴漿菌仍可存活一段時間，而水浴槽更是嚴重的污染源，黴漿菌甚至可存活於純水中。

一般而言，我們使用HEPA濾膜過濾無菌操作台之空氣，黴漿菌雖不像孢子一樣會隨風飄散，但仍有可能直接污染通風管、風扇等而直接進入無菌操作台。

加熱法一直是有效去除黴漿菌污染的方法，另也有人使用含甲醛的藥劑來去除黴漿菌。但針對黴漿菌、細菌或病毒選擇 T-Pro MycoClean spray 是可以有效去除的噴劑，噴劑使用後約2~3分鐘即可以拭紙擦除或等待10分鐘使其自然揮發。

特色：

  • 可快速去除黴漿菌
  • 噴劑型簡單方便操作
  • 非腐蝕性及致癌性
  • 適用於實驗室各設備器材表面

說明：

T-Pro MycoClean spray 是可以有效去除黴漿菌污染，同時它對細菌、病毒也有極佳的清潔效果(如Staphyhococcus aureus、Streptococcus faecalis、Escherichia coli、Proteus mirabilis、Pseudomonas aeruginosa、Candida albicans、HBV、Papova-、Adeno-、Polio- and Vaccinia Viridae)，T-Pro MycoClean spray是極佳的抑菌、清潔、去污噴劑一次搞定。

需求請洽萊恩生物科技有限公司服務人員
公司電話: 04-25239639
業務專員: 陳俊豪
行動電話: 0937-229803
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